PAX8 (M)

Product Description

PAX8 Antibody

PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins which contain a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. PAX8 is involved in kidney cell differentiation, thyroid development, or thyroid dysgenesis.

PAX8 is expressed in a high percentage of renal cell carcinomas and ovarian cancers. This mouse monoclonal PAX8 antibody [BC12] has been designed to target a restricted epitope and exhibits higher specificity and provides sharper staining than the PAX8 rabbit polyclonal antibody. Unlike the polyclonal PAX8, this mouse monoclonal antibody does not stain B-cells and does not recognize epitopes of pancreatic origin and neuroendocrine cells in stomach and colon; thus, providing superior specificity. The expression of the mouse monoclonal PAX8 target antigens was found in normal kidney, thyroid, and cervix, but was not identified in normal ovary. By Western blot, [BC12] has been shown to recognize PAX8 and not PAX2, PAX5 or PAX6 proteins.

PAX8 stains nuclei exclusively and performs well in formalin-fixed paraffin-embedded tissues. U.S. Patents 8,852,592, 9,417,243, and patents pending.

Specifications

Regulatory Notice: Biocare’s IVD-labeled products comply with US-FDA and European IVDD regulation. Other regions may have additional requirements for such labeling, please contact your local distributor.

1. Tacha D, Qi W, Zhou D, Bremer R, Cheng L. PAX8 Mouse Monoclonal Antibody [BC12] Recognizes a Restricted Epitope and Is Highly Sensitive in Renal Cell and Ovarian Cancers But Does Not Cross-react With B Cells and Tumors of Pancreatic Origin. Appl Immunohistochem Mol Morphol. Published Ahead-of-Print, May 16, 2012.
2. Tacha D, Zhou D, Cheng L. Expression of PAX8 in Normal and Neoplastic Tissues: A Comprehensive Immunohistochemical Study. Appl Immunohistochem Mol Morphol. 2011 Jul;19(4):293-9.
3. Lotan TL, Ye H, Melamed J, Wu XR, Shih IM, Epstein JI. Immunohistochemical panel to identify the primary site of invasive micropapillary carcinoma. Am J Surg Pathol. 2009 Jul; 33(7):1037-41.
4. Viktorová T, Babjuk M, Dusková J, Stolz J, Goetz P, Mares J. Expression of PAX2 and PAX8 genes in conventional type of renal carcinoma and their role in the tumor prognosis. Diagn Cytopathol. 2008 Aug; 36(8):568-73.
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6. Moretti L, Medeiros LJ, Kunkalla K et. al. N-terminal PAX8 polyclonal antibody shows cross-reactivity with N-terminal region of PAX5 and is responsible for reports of PAX8 positivity in malignant lymphomas. Mod Pathol 2012 Feb;25(2):231-6.
7. Lorenzo PI, Jimenez Moreno CM, Delgado I et. al. . Immunohistochemical assessment of Pax8 expression during pancreatic islet development and in human neuroendocrine tumors. Histochem Cell Biol 2011 Nov;136(5):595-607.
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