The 100/D5 antibody is highly specific to Bcl-2 antibody (alpha) and shows no cross-reactivity with Bcl-x or Bax protein. Bcl-2 (b-cell lymphoma #2) is a proto-oncogene located at 18q21.3. Expression of Bcl-2 alpha oncoprotein has been shown to inhibit apoptosis. In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 protein, whereas the normal or hyperplastic germinal centers are negative. Various B- and T-cell lymphoproliferative diseases and some diffuse large B-cell lymphomas are Bcl-2 positive while Burkitt’s lymphoma/leukemia is generally negative.
1. Korsmeyer SJ. Cancer Res. 1999 Apr; 59(7 Suppl):1693s-1700s. 2. Snuderl M, et al. Am J Surg Pathol. 2010 Mar; 34(3):327-40. 3. Alderson LM, et al. Cancer Res. 1995 Mar; 55(5):999-1001. 4. Symmans WF, et al. Acta Cytol. 1995 Jul-Aug; 39(4):673-82. 5. Triscott JA, et al. J Cutan Pathol. 1995 Feb; 22(1):2-10.