Melanoma Cocktail

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Melanoma antibody Cocktail is a combination of HMB45 and MART-1. HMB45 has been shown to react with cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells, labeling the majority of melanomas. MART-1 has been shown to label melanomas and tumors showing melanocytic differentiation. Studies have also shown that MART-1 is more sensitive than HMB-45 when labeling metastatic melanomas. HMB45 and MART-1 are coexpressed in the majority of melanomas, as well as solely expressed in certain cases. Thus, a HMB45 and MART-1 cocktail has been reported to be a potentially sensitive first-order pan melanoma screener.

Intended Use

IVD

Species Reactivity

Human

Source

Mouse Monoclonal

Clone

HMB45 + M2-7C10 + M2-9E3

Isotype

IgG2b + IgG2b

Antigen

HMB45 and MART-1

Localization

Cytoplasmic

Positive Control

Metastatic melanoma in lymph node

1. Blessing K, Sanders DS, Grant JJ. Comparison of immunohistochemical staining of
the novel antibody Melan-A with S100protein and HMB-45 in malignant melanoma
and melanoma variants. Histopathology 1998 Feb; 32 (2):139-146.
2. Jungbluth AA et al. A103: An anti-melan-a monoclonal antibody for the detection
of malignant melanoma in paraffin-embedded tissues. Am J Surg Pathol 1998 May;22
(5):595-602.
3. Beaty MW et al. Effusion cytology of malignant melanoma: A morphologic and
immunocytochemical analysis including application of the MART-1 antibody.
Cancer 1997 Feb 25;81(1):57-63.
4. Bonetti F, et al. False-positive immunostaining of normal epithelia and carcinomas
with ascites fluid preparations of antimelanoma monoclonal antibody HMB45. Amer
J. clin Pathol 1991;95:454
5. Leong AS-Y, Millos J. An assessment of a melanoma-specific antibody(HMB-45)
and other immunohistochmical markers of malignant melanoma in paraffin-embedded
tissues. Surg Pathol 1989;2:137.
6. Ordonez NG, et al. Comparison of HMB45 monoclonal antibody and S100 protein
in the immunohistochemical diagnosis of melanoma. Amer J Clin Pathol 1988;90:385.
7. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22,
Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove
Azide Salts.”
8. National Committee for Clinical Laboratory Standards(NCCLS). Protection of
laboratory workers from infectious diseases transmitted by blood and tissue; proposed
guideline. Villanova, PA 1991;7(9). Order code M29-P.

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