HIER Decloaking Chamber™

Decloaking Chamber™ NxGen

The Decloaking Chamber™ NxGen has been designed for heat-induced epitope retrieval (HIER) and ease of use. It has 5 discrete temperature settings ranging between 60ºC and 110ºC with user programmable times. The 110ºC antigen retrieval protocol can be completed from start to finish in under an hour. With a 72 total slide capacity and only minutes of hands-on time per run, the Decloaking Chamber NxGen offers a walk-away capability similar to fully automated staining instruments.

The Decloaking Chamber NxGen transfers run data to a USB drive for export to a user’s computer. The run data recorded includes the date and time per run with temperature and pressure readings throughout. With the Decloaking Chamber NxGen recalling the settings from the last run, a quick start of the same protocol is possible.

The Decloaking Chamber NxGen is an excellent instrument for HIER. The proper use of heat and pressure in conjunction with the appropriate buffer solutions is of the utmost importance for consistent immunohistochemistry (IHC) staining. The Decloaking Chamber NxGen is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety and quality control requirements. Temperature, pressure and time can be monitored and recorded with the Decloaking Chamber NxGen to produce consistent staining.

Buy Decloaking Chamber™ NxGen

CE Listed, ETL approved, U.S. patent number 6,580,056, June 17, 2003

heat induced epitome retrieval machine

Standardized Results

  • Crisp and even staining
  • Very gentle on tissues
  • Reproducible results
  • Reduction of false negatives

Quality Controlled System

  • pH indicators change color with temperature
  • Two-step deparaffinization and retrieval buffers
  • Steam monitor strips for temperature verification
  • Audible alert upon run completion

Ordering Information

Decloaking Chamber Description Cat. No. Volume
Decloaking Chamber™ NxGen (For use in 110 V markets) DC2012 1 ea.
Decloaking Chamber™ NxGen (For use in 220 V markets) DC2012-220V 1 ea.

For pricing and availability of our instrumentation, please contact us.

Size 14.2” x 13.5” x 13” / 36.1 cm x 34.3 cm x 33.0 cm
Weight 13 lbs / 6.91 kg
Temperature Range 60 ºC – 110 ºC (+/- 5 ºC)
Slide capacity 72 total slides (3 slide canisters of 24 slides each)
Power Requirements  115V/60Hz/1000W or 230V/50Hz/1000W


Basket, Rack Holder

$1,003.00Add to cart

Metal Slide Canister

$365.00$741.00Select options

Kling-On Microscope Slides

$1,188.00Select options

Sealing Gasket Kit

$684.00Add to cart

Condensation Collector

$73.00Add to cart

Flash Drive with Software & Manual

$307.00Add to cart

Ancillaries for Decloaking Chamber 2002/08

Kling-On Microscope Slides

$1,188.00Select options

Tissue Tek Slide Holders and Containers

$1,045.00Add to cart

Decloaker Gasket

$192.00Add to cart

Steam Monitor Strips for Quality Control for all Decloaking Chamber Models

The steam monitor strips are heat and pressure sensitive, acting as a quality control check and confirms that the chamber has reached full temperature and pressure as evidenced by the dark brown to black color. The strips can be placed in your laboratory notebook as a permanent record for that run.

pH Strips for Quality Control for all Decloaking Chamber Models

The pH of citrate-based retrieval solutions increase as the temperature rises. To assure quality control and consistent IHC staining, Biocare has developed heat-tested pH strips that give accurate and the true pH at high temperatures. The pH of a solution should be recorded at the temperature used. Using these pH strips, the laboratory investigator can now record pH on a daily basis, and comply with CAP and JCOH inspection.

Steam Monitor Strips

$727.00Select options

QC pH Strips pH 4-7

$147.00Add to cart

QC pH Strips pH 7.5-10.5

$161.00Add to cart

This quick and informative video series shows how easy it is to unpack and operate the Decloaking Chamber NxGen.

Decloaking Chamber NxGen New Installation

With only a few minutes of unpacking, the DC NxGen will be ready to perform heat induced epitope retrieval (HIER) on microscope slides. This video shows what is in the package and how to unpack the unit.

Decloaking Chamber NxGen Set-up and Loading

See how easy it is to operate the Decloaking Chamber NxGen. The video below demonstrates the quick set-up and proper loading of the Decloaking Chamber NxGen in order to perform heat induced epitope retrieval (HIER) on microscope slides.

Decloaking Chamber NxGen Run Reporting

The Decloaking Chamber NxGen comes with advanced easy-to-use run reporting software. See how to use the software and what reports you can create from the Decloaking Chamber NxGen data.