- 20 – 40 times more sensitive than conventional conjugated mouse adsorbed anti-rat HRP-secondary detection
- Use rat primary antibodies on mouse tissues
- mouse adsorbed anti-rat detection systems display minimum cross-reactivity to endogenous mouse IgG
What Tissues Are You Working With?
Mouse Primary Antibodies
This novel detection technology allows for use of mouse primary antibodies on mouse tissues. Available as a bundle consisting of detection micro-polymer, blocking and HIER solution or available individual components.
Mouse-on-Mouse: Mouse Hepatocyte Specific Antigen on mouse liver
Use with mouse primary antibodies on rat tissues. Available as a bundle containing detection polymer, blocking and HIER solution or available individually.
Mouse-on-Rat: Mouse Neurofilament on rat brain
Rodent Detection Ordering Information
Rat Primary Antibodies
Rat-on-Mouse Micro-polymer Detection
Use with rat monoclonal antibodies on mouse tissues. The Rat-on-Mouse HRP / AP micro-polymer detection is a two-step system. It is comprised of two reagents: a rat probe that binds to the rat primary antibody; and an HRP / AP micro-polymer that conjugates to the probe.
Rat Detection Ordering Information
Rabbit Primary Antibodies
Rabbit-on-Rodent Micro-polymer Detection
Rabbit-on-Rodent detection is available as a micro-polymer only.
Rabbit Detection Ordering Information
Goat-on-Rodent Primary Antibodies
Goat-on-Rodent Micro-polymer Detection
Use with goat primary antibodies on mouse, rat and human tissues. The goat detection is a two-step system.
- 25 – 50 times more sensitive than conventional mouse anti-goat HRP secondary detection systems
- 10 – 20 times more sensitive than conventional mouse anti-goat AP secondary detection systems
- Goat secondary detections display minimum cross-reactivity to endogenous mouse & rat IgG
Goat anti-E-cadherin on mouse liver (DAB)
Goat anti-E-cadherin on mouse liver (FR)
Goat Detection Ordering Information
Retrieval and Blocking Reagents
Blocking & HIER reagents to enhance specificity & reduce / eliminate background
This antigen retrieval buffer is specially formulated to work with Biocare’s Promark detection systems. Rodent Decloaker with proprietary blocking reagents help reduce mouse or rat endogenous IgG in rodent tissue. This is particularly useful for brain tissue; helps reduce nonspecific background staining and performs antigen retrieval. Antibody titers can be doubled or tripled when using Rodent Decloaker compared to the use of citrate buffer.
Rodent Block R
This blocking reagent, designed for use exclusively with rat tissue, makes use of proprietary blocking agents to reduce and/or eliminate endogenous rat IgG. To be used with mouse-on-rat, rabbit-on-rodent and goat micro-polymer detection systems.
Rodent Block M
This blocking reagent, designed for use exclusively with mouse tissue, makes use of proprietary blocking agents to reduce and/or eliminate endogenous mouse IgG. To be used with mouse-on-mouse, rat-on-mouse, rabbit-on-rodent and goat micro-polymer detection systems.
This blocking reagent is a potent blocker for eliminating endogenous rat IgG in rat tissues. The combination of Rodent Block R and XR Factor allows primary goat, rabbit and mouse antibodies to be used on rat tissues. XR Factor is formulated for use with mouse, rabbit, or goat micro-polymer detection systems, but should not be used with the rat micro-polymer detection system.
This blocking reagent is a potent blocker for eliminating endogenous mouse IgG in mouse tissues. The combination of Rodent Block M and XM Factor allows primary goat, rabbit and mouse antibodies to be used on mouse tissues. XM Factor is formulated for use with rat, rabbit or goat micro-polymer detection reagents, but should not be used with any mouse micro-polymer detection systems.
Biocare’s Rodent Decloaker, Rodent Block M and R, XR Factor and XM Factor can also be used with Biocare’s Fluorescent DyLight kits. Biocare DyLight fluorescent detection reagents may be used on mouse and rat tissues by adding 1-2 drops the XR or XM Factor to the fluorescent-secondary detection reagent. Use Rodent Block M and R prior to application of the primary antibody for optimal results.
Reduced background staining with use of Rodent Block and XR Factor
Arrows point out endogenous background due to staining of IgG.
Mouse liver without Rodent Block and XR Factor
Mouse liver with Rodent Block and XR Factor