Rabbit-on-Rodent HRP-Polymer is a one-step micro-polymer detection developed for use with rabbit monoclonal/polyclonal antibodies on mouse and rat tissue. Rabbit primary antibodies on mouse and rat tissues are advantageous because rabbit secondary detection systems can be used with minimum cross-reactivity to endogenous mouse and rat IgG. Biocare Medical has developed a Rabbit HRP-Polymer detection system with minimal cross-reactivity to endogenous mouse and rat IgG. The advantages of this polymer technology are increased sensitivity, reduction of IHC steps and the elimination of endogenous biotin. It can be used with formalin-fixed paraffin-embedded tissues, floating sections, frozen sections and cell culture. Biocare’s new rabbit polymer is ideal for single staining and can also be used in double and triple staining techniques. In some tissues such as spleen, endogenous mouse or rat IgG may be difficult to eliminate. Biocare Medical has developed an antigen retrieval solution (Rodent Decloaker) that helps to reduce or eliminate endogenous IgG and non-specific background staining, and at the same time, performs antigen retrieval. Temperature dependent protocols can be used at 80, 95 and 125ºC using Biocare’s Decloaking Chamber. In addition, Biocare Medical has introduced new products to help eliminate persistent rat and mouse IgG. The XM and XR Factor are two potent blockers for eliminating endogenous mouse IgG and rat IgG, respectively. By adding 1-2 drops (1 drop equals 33µl) of the XM or XR Factor to 2.5 ml of rabbit polymer detection, endogenous mouse and/or rat IgG will be reduced dramatically or completely eliminated. Biocare has also developed Rodent Block M and Rodent Block R that block for non-specific background staining, and at the same time, reduce endogenous mouse and rat IgG, respectively. Rodent Block M or R is applied prior to the primary antibody for 15 to 30 minutes.
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