The Mouse-on-Mouse AP-Polymer is specially designed for using mouse primary antibodies on mouse tissues with minimum cross-reactivity to endogenous mouse IgG. Utilization of this goat anti-mouse alkaline phosphatase (AP) polymer is advantageous over traditional biotinylation methods. Biotinylated anti-mouse secondary antibodies can bind to endogenous mouse IgG in the tissue in addition to the primary antibody, leading to troublesome background staining. Biocare Medical’s biotin-free polymer technology provides the elimination of endogenous biotin, reduction of IHC steps and increased sensitivity.
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