P504S, also known as α-methylacyl coenzyme A racemase (AMACR), is a peroxisomal and mitochondrial enzyme that plays a role in bile acid synthesis and β-oxidation of branched chain fatty acids. P504S was initially identified from a cDNA library as a gene that is overexpressed in human prostate cancer; with little or no expression in normal prostate. In immunohistochemistry, P504S (P) antibody has been shown to be a specific marker of prostatic adenocarcinoma. Additionally, prostate glands involved in PIN have been found to express P504S, whereas P504S was nearly undetectable in benign glands.
1. Ferdinandusse S, et. al. Subcellular localization and physiological role of α-
methylacyl-CoA racemase. J Lipid Res. 2000; 41:1890-6.
2. Xu J, et. al. Identification of Differentially Expressed Genes in Human Prostate
Cancer Using Subtraction and Microarray. Cancer Res. 2000; 60:1677-82.
3. Rubin MA, et. al. α-Methylacyl Coenzyme A Racemase as a Tissue Biomarker for
Prostate Cancer. JAMA. 2002; 287:1662-70.
4. Luo J, et. al. Alpha-methylacyl-CoA racemase: a new molecular marker for prostate
cancer. Cancer Res. 2002; 62:2220-6.
5. Zhou M, et. al. Alpha-Methylacyl-CoA Racemase A Novel Tumor Marker
Overexpressed in Several Human Cancers and Their Precursor Lesions. Am J Surg
Pathol. 2002; 26:926-31.
6. Wu CL, et. al. Analysis of α-Methylacyl-CoA Racemase (P504S) Expression in
High-Grade Prostatic Intraepithelial Neoplasia. Hum Pathol. 2004; 35:1008-13.
7. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22,
Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove
8. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory
Workers from Occupationally Acquired Infections; Approved guideline-Third Edition
CLSI document M29-A3 Wayne, PA 2005.