A simple in situ hybridization technology using proprietary DNA
probes for rapid visualization of mRNA target expression.
The RISH™ probe technology, enables extremely stable hybridization with the mRNA target, resulting in a more abundant signal and conferring highly specific staining.
The protocol has been simplified by removal of the overnight hybridization, requirement for RNase free solutions, labware and harsh stringent wash conditions resulting in a procedure that is completed in approximately 2.5 hours.
The result is clear, with virtually no background; the chromogenic signal is easily visualized under brightfield microscopy, along with the tissue morphology on a single slide.
|RISH™ Epstein-Barr Encoded RNA (EBER) Probe||RI0001 T||ASR | RUO|
|RISH™ Kappa Light Chain DNA Probe||RI0004 T||ASR | RUO|
|RISH™ Lambda Light Chain DNA Probe||RI0005 T||ASR | RUO|
|RISH™ Cytomegalovirus (CMV) Probe||RI0011 T||ASR | RUO|
|RISH™ Dual Kappa/Lambda Probe||RI0027 T||Data Sheet|
|RISH™ Retrieval, 10X||RI0209 M||Data Sheet|
|RISH™ AP Detection Kit||RI0213 KG||Data Sheet|
|RISH™ HRP Detection Kit||RI0207 KG||Data Sheet|
|RISH™ Dual Detection Kit||RI0208 KG||Data Sheet|
|RISH™ HybriSlips™||Cat. No.||Download|
|RISH™ HybriSlips™ 22mm x 22mm||RI0210 C (100 coverslips)||Data Sheet|
|RISHzyme™ Buffer||6 ml|
|RISH™ Secondary Reagent||6 ml|
|RISH™ HRP Tertiary Reagent||6 ml|
|Betazoid DAB (Diaminobenzidine) Chromogen||0.25 ml|
|Betazoid DAB Buffer||6 ml|
|DAB Sparkle||6 ml|
|* RISH Dual Detection Kit includes Vulcan Fast Red chromogen, buffer & additional mixing vial|