RISH™

• About
• Light Chains
• EBER
• CMV
• Ordering Information

A simple in situ hybridization technology using proprietary DNA
probes for rapid visualization of mRNA target expression.

• Rapid – Results in approximately 2.5 hours
• Simple Procedure – 5 step protocol is as simple as IHC
• Sensitive – Proprietary DNA technology designed for enhanced sensitivity & specificity
• Easy – Simultaneously evaluate gene expression and tissue morphology
• Archivable – Chromogenic signal is stable for extended storage

The RISH™ probe technology, enables extremely stable hybridization with the mRNA target, resulting in a more abundant signal and conferring highly specific staining.

The protocol has been simplified by removal of the overnight hybridization, requirement for RNase free solutions, labware and harsh stringent wash conditions resulting in a procedure that is completed in approximately 2.5 hours.

The result is clear, with virtually no background; the chromogenic signal is easily visualized under brightfield microscopy, along with the tissue morphology on a single slide.

Immunoglobulin Light Chains – Kappa and Lambda

• Differentiate immunoblastic reactions related to viral infections, such as mononucleosis, from lymphoid tumors
• Used in the study of monoclonality of lymphoid tumors, lymphoproliferative syndromes, myelomas and immunodeficiency associated lymphoproliferative syndromes
• Diagnose mono- or polyclonality in lymphoid tumor proliferation or reactive processes

Dual Kappa / Lambda Probe

• The Multiplex Dual Kappa-Lambda probe set enables simultaneous evaluation of immunoglobulin light chain ratios in one tissue section

EBER (Epstein-Barr virus Encoded RNA)

• Detects Epstein-Barr Virus in Reed-Sternberg (RS) Cells
• Used to diagnose nasopharyngeal carcinoma
• Used to diagnose infectious mononucleosis, lymphoid/non-lymphoid tumors associated with Epstein-Barr viral infection

Cytomegalovirus (CMV) Probe

• Detects CMV genomic DNA & mRNA in infected tissues or cells
• CMV is a member of the human herpes virus-5 (HHV-5) group transmited in breast milk, during organ transplantation, sexual activity or blood transfusions

Ordering Information

Detection Kit Components