MACH 3™

MACH 3 is a two-step, biotin-free detection system which provides excellent specificity, sensitivity and nuclear staining for mouse or rabbit primary antibodies.

MACH 3 Features

  • A sensitivity-enhancing secondary reagent
  • Increased density of enzymes bound to tertiary target
  • 5- to 10- fold increased staining sensitivity when compared to conventional dextran polymer detection kits
  • Superior staining for nuclear and cytoplasmic/cell surface antigens
  • Elimination of background
  • High primary antibody dilution significantly improves specificity and reduces cost
  • Adapted for all Biocare’s prediluted antibodies
  • Compatible with automated immunostainers

Compare MACH 3 (left) with leading competitor (right) for Ki-67
MACH 3’s superior sensitivity allows clean and strong staining, even at higher primary antibody dilution factors.

MACH 3, 1:200 dilutionCompetitor: Dextran backbone polymer, 1:200 dilution

Ordering Information

Biocare offers MACH 3 Detection Kits for either mouse or rabbit primary antibodies, labeled with either Horseradish Peroxidase (HRP) or Alkaline Phosphatase (AP).

Product Name Description Cat. No. Download
MACH 3 Mouse HRP Polymer Detection Mouse Probe, Mouse Micro-polymer M3M530 G, H, L Data Sheet
MACH 3 Mouse AP Polymer Detection Mouse Probe, Mouse Micro-polymer M3M532 G, H, L Data Sheet
MACH 3 Rabbit HRP Polymer Detection Rabbit Probe, Rabbit Micro-polymer M3R531 G, H, L Data Sheet
MACH 3 Rabbit AP Polymer Detection Rabbit Probe, Rabbit Micro-polymer M3R533 G, H, L Data Sheet