Heat-induced epitope retrieval methods (HIER) are used to pretreat tissues for optimum antibody-antigen reaction. Many different kinds of heat retrieval methods are used. These include the following: microwave, waterbath, steamers and pressure cooker methods. These methods were developed to “Standardize” immunohistochemistry for formalin-fixed, paraffin-embedded tissues. Heat retrieval methods’ lack of “standardization” and quality control (QC) procedures is a problem. BIOCARE QC Steam Monitor Strips are for antigen retrieval in a pressure steam chamber (Decloaking Chamber). The Steam Monitor Strip gives a visual QC to make sure that the pressure cooker has reached the optimum temperature and pressure.
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