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The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. IH8 was found to be reactive with purified major capsid protein (MCP). The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein, as a target for screening. This antibody reacted with a 56 kilodalton protein in cells infected with L1-vaccinia virus, and the protein was present in HPV16. A panel of p16 and Ki-67 can be used for further evaluation. CAMVIR-1 may also be used separately for phenotyping HPV-16. Other HPV isotypes may also be reactive with the Broad Spectrum HPV antibody, but have not been tested.
BPV-1/1H8 + CAMVIR-1
IgG + IgG2a
HPV Cocktail Broad Spectrum (HPV-1, 6, 11, 16-16, 18 and 31)
Infected cervical biopsy
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1. Cowsert LM, Pilacinski WP, Jenson AB. Identification of the bovine papillomavirus L1 gene product using monoclonal antibodies. Virology 1988 Aug;165(2):613
2. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
3. National Committee for Clinical Laboratory Standards(NCCLS). Protection of laboratory workers from infectious diseases transmitted by blood and tissue; proposed guideline. Villanova, PA 1991;7(9). Order code M29-P.