In normal epithelia, HMW Cytokeratins (CK5 and CK14) stain stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The p63 is detected in prostate basal cells in normal prostate, however, is negative in malignant tumors of the prostate gland. Thus p63 has been shown to be useful as a differential marker for benign and malignant tumors of prostate gland and can be useful as a negative marker. The combination of the HMW CK Cocktail and p63, HMW CK + p63 antibody has been shown to be superior to each alone.
1. Tacha DE, Miller RT. Use of p63/P504S monoclonal antibody cocktail in immunohistochemical staining of prostate tissue. Appl Immunohistochem Mol Morphol. 2004 Mar;12(1):75-8.Biocare Medical, Walnut Creek, California.
2. Signoretti S, Waltregny D, et al. p63 is a prostate basal cell marker and is required for prostate development. Am J Pathol 2000 Dec; 157(6):1769-75.
3. Wang Y, et al. Cell differentiation lineage in the prostate. Differentiation. 2001 Oct;68(4-5):270-9.
4. Tokar EJ, et al. Stem/prodenitor and intermediate cell types and the origin of human prostate cancer. Differentiation. 2005 Dec;73(9-10):463-73.
5. Collins AT, et al. Identification and isolation of human prostate epithelial stem cells based on alpha(2)beta(1)-integrin expression. J Cell Sci.2001 Nov;114(Pt 21):3865-72.
6. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
7. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved guideline-Third Edition CLSI document M29-A3 Wayne, PA 2005.