Clones EP17 and EP30 recognize cytokeratins (CK) 8 and 18, both of which are low molecular weight intermediate filament proteins (52.5 kDa and 45 kDa respectively). In normal tissues, CK8/18 recognizes all simple and glandular epithelium (1). In neoplastic tissues, CK8/18 may prove useful for the identification of most adenocarcinomas and some squamous cell carcinomas. CK8/18 expression patterns may also aid in the classification of tumors of unknown origin and poorly differentiated carcinomas (1-3). CK8/18 rabbit monoclonal may be useful as a staining mask in a multiplex stain with mouse monoclonal antibodies such as Ki-67, PD-1 or CD8. As CK8/18 does not stain stromal components or tumor infiltrating lymphocytes in various carcinomas such as breast or colon cancers, a CK8/18 staining mask may be helpful with these applications.
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3. Makino T, et al. Cytokeratins 18 and 8 are poor prognostic markers in patients with squamous cell carcinoma of the oesophagus. Br J Cancer. 2009 Oct 20;101(8):1298 -306.
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