Want to Swap Avidin for Streptavidin? 4Plus Your Results When You Switch
There are several advantages when using streptavidin versus an avidin complex (ABC). In contrast to avidin, streptavidin is not glycosylated and is therefore uncharged at neutral pH (6.5 versus 10), reducing nonspecific background staining. Streptavidin also lacks the carbohydrate side chains that may be an additional cause of non-specific background. Another key advantage of streptavidin is the significant increase in sensitivity (perhaps from less steric hindrance), thus facilitating an increase in overall binding capacity. Streptavidin-enzyme conjugates are also more stable than an ABC complex. The ABC complex must be freshly made 30 minutes prior to use and is stable only for a few days, while a streptavidin-conjugate can be stored for up to 1-2 years and is ready to use, saving time and room for error. To learn more about switching from Avidin to Streptavidin download your free whitepaper below!
Streptavidin significantly increases sensitivity, facilitating greater binding capacity
Streptavidin protein can bind up to four biotin molecules, which are normally conjugated to an enzyme, antibody or target protein
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Mouse Smooth Muscle Actin with B- and T-cells