4plus™


A sensitive two-step HRP and AP detection method

Biocare’s 4plus detection systems are designed for reliable, cost-effective, two-step detection, providing a high-level of sensitivity. The vast majority of primary antibodies can be diluted two-fold compared to other commercially available avidin-biotin detection systems. Biocare’s prediluted antibodies are all standardized to work with the 4plus detection system.

4plus detection system can be used with mouse and rabbit primary antibodies. After labeling the antigen with primary antibody, a species-specific or universal, affinity-purified, biotinylated secondary antibody is added to bind. Horseradish peroxidase (HRP) or alkaline phosphatase (AP) labeled-streptavidin is then added. A chromogen/substrate is applied that reacts with a HRP or AP to produce an intense color signal.

Mouse-on-Mouse Biotinylation Kit: Mouse Smooth Muscle Actin with B- and T-cellsMouse Smooth Muscle Actin with B- and T-cells

Biocare’s Avidin-Biotin Blocking Kits are optimized for blocking endogenous biotin in tissue sections. Endogenous biotin is abundant in cells such as hepatocytes, kidney tubules and many tumors, particularly carcinomas. Biocare’s biotin-blocking kits can eliminate nonspecific false-positive staining when using avidin-biotin detection systems. The blocking step is particularly critical when using heat-induced epitope retrieval (HIER), which unmasks endogeneous biotin in tissues. (CAP checklist question ANP.22615)

Used with both mouse and rabbit primary antibodies. 4plus Universal HRP and ALP and 4plus species specific components are also available as bulk reagents.

Ordering Information

4-plus Detection Kits
4plus HRP Universal Detection

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4 Plus Biotinylated Secondary Antibodies
Universal Goat Link

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Goat Anti-Mouse IgG

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Goat Anti-Rabbit IgG

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Goat Anti-Rabbit IgG

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4 Plus Streptavidin-Enzyme Conjugates
4plus Streptavidin AP Label

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4plus Streptavidin HRP Label

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