ONCORE Pro CytoFISH Kit contains reagents used in the pretreatment of cytological specimens to enhance probe accessibility to nucleic acid targets as well as a post-hybridization buffer used to eliminate nonspecific binding of the probe.
Cytological specimens with FISH protocols usually require an enzymatic pretreatment to achieve proper staining. FISHzyme is a commonly used pepsin digestive enzyme. Enzyme pretreatment helps probe accessibility to nucleic acid targets. 1% Formaldehyde diluted in PBS buffer acts as a fixative to better preserve cell morphology. Ethanol, 70% is necessary for tissue dehydration before a probe application. SSC Wash Buffer is a ready-to-use saline sodium citrate buffer (pH 7.0- 7.5) that contains 0.3% NP40 and Proclin 950 as a preservative. SSC Wash Buffer contains 30 mM citrate, which is commonly identified as 2X SSC. Additional wash buffers 2XSSC/0.1%NP-40 and 0.4XSSC/0.3%NP-40 are utilized at different steps of the protocol before or after probe hybridization.