ONCORE Pro CytoFISH Kit

Product Description

ONCORE Pro CytoFISH Kit contains reagents used in the pretreatment of cytological specimens to enhance probe accessibility to nucleic acid targets as well as a post-hybridization buffer used to eliminate nonspecific binding of the probe.

Cytological specimens with FISH protocols usually require an enzymatic pretreatment to achieve proper staining. FISHzyme is a commonly used pepsin digestive enzyme. Enzyme pretreatment helps probe accessibility to nucleic acid targets. 1% Formaldehyde diluted in PBS buffer acts as a fixative to better preserve cell morphology. Ethanol, 70% is necessary for tissue dehydration before a probe application. SSC Wash Buffer is a ready-to-use saline sodium citrate buffer (pH 7.0- 7.5) that contains 0.3% NP40 and Proclin 950 as a preservative. SSC Wash Buffer contains 30 mM citrate, which is commonly identified as 2X SSC. Additional wash buffers 2XSSC/0.1%NP-40 and 0.4XSSC/0.3%NP-40 are utilized at different steps of the protocol before or after probe hybridization.

Specifications

1. Analysis of genes and chromosomes by nonisotopic in situ hybridization. Lichter P, et al. Genet Anal Tech Appl. 1991 Feb;8(1):24- 35.
2. Fluorescence in situ Hybridization (FISH). Bayani J, Squire JA. Curr Protoc Cell Biol. 2004.
3. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition (M29-A4) Wayne, PA 2014.