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Studies have shown that the Microglia antibody, also known as Iba1 (ionizing calcium-binding adaptor molecule 1), is a novel protein that it is specifically expressed in macrophages/microglia and is upregulated during the activation of these cells. Studies have shown cross-reactivity in human, mouse and rat tissues. Glial Fibrillary Acidic Protein (GFAP) and Microglia antibodies have been used as markers for axonal damage, reactive astrocytes and activated microglia, respectively. The Iba1 polyclonal antibody does not cross-react with neurons or astrocytes.

Species Reactivity

Human, Mouse, Rat


Rabbit Polyclonal








Cytoplasm of microglia and macrophages

Positive Control

Normal brain*

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1. Ito D, Imai Y, Ohsawa K, Nakajima K, Fukuuchi Y, Kohsaka S. Microglia-specific localization of a novel calcium binding protein, Iba1. Brain Res Mol Brain Res. 1998 Jun 1;57(1):1-9.
2. Okere CO, Kaba H. Heterogenous immunohistochemical expression of microgliaspecific ionized calcium binding adaptor protein (Iba1) in the mouse olfactory bulb. Brain Res. 2000 Sep 15;877(1):85-90.
3. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
4. National Committee for Clinical Laboratory Standards (NCCLS). Protection of laboratory workers from infectious diseases transmitted by blood and tissue; proposed guideline. Villanova, PA 1991;7(9). Order code M29-P.


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