Monoclonal antibody to human CD3, when used in conjunction with other antibodies, is regarded as a reliable pan T-cell antibody for the immunophenotyping of lymphomas in paraffin sections. Most T-cell lymphomas show positivity for CD3 with exception of the more aggressive large T-cell lymphomas and anaplastic large cell (CD30) lymphomas. CD3 immunoreactivity has also been reported in a minority of Reed-Sternberg cells of Hodgkin’s disease and in some histiocytic tumors. Studies suggest that CD3 with UCHL-1 identified the majority of T-cell lymphomas in paraffin sections.
1. Cabecadas JM, Isaacson PG. Histopathology. 1991 Nov; 19(5):419-24.
2. Steward M, et al. Histopathology. 1997 Jan; 30(1):16-22.