Detection 4plus

Biocare’s 4plus detection systems are designed for reliable, cost-effective, two-step detection, providing a high-level of sensitivity. The vast majority of primary antibodies can be diluted two-fold compared to other commercially available avidin-biotin detection systems. Biocare’s prediluted antibodies are all standardized to work with the 4plus detection system.

4plus detection system can be used with mouse and rabbit primary antibodies. After labeling the antigen with primary antibody, a species-specific or universal, affinity-purified, biotinylated secondary antibody is added to bind. Horseradish peroxidase (HRP) or alkaline phosphatase (AP) labeled-streptavidin is then added. A chromogen/substrate is applied that reacts with a HRP or AP to produce an intense color signal.

^{Mouse Smooth Muscle Actin with B- and T-cells}