Proper optimization is vital for successful immunohistochemical (IHC) testing. An optimal IHC protocol can be characterized by its ability to consistently and unambiguously determine how the antigen is expressed in the tissue. Optimization is fundamental to reducing the risk of both false positive and false negative results.1 The protocol must be able to identify both […]

Regular cleaning is an essential part of instrument maintenance, but what happens if you don’t have Lysol? Following recommended cleaning procedures to remove residues and sterilize instrument components will improve instrument performance and help extend the instrument’s lifespan. This white paper looks at Lysol alternatives for cleaning to ensure your automation platform keeps running perfectly! […]

As anyone who has worked in histology will tell you, a successful staining process begins long before the staining protocol itself. In fact, factors that will determine a stain’s success or failure start to come into play soon as the tissue is removed from the patient’s body. As such, proper education and training for all […]

Validation is an essential step for setting up a new laboratory procedure, whether it incorporates immunohistochemical (IHC) or routine ematoxylin and eosin (H&E) staining. The validation process involves repeatedly testing the procedure over a large quantity and range of specimens in order to ensure the procedure will yield consistent and reproducible results. This, in turn, […]

Tissue microarrays (TMAs) are paraffin blocks that include multiple samples from different tissue types meant to provide a comprehensive multiplex staining profile. The sections from TMA blocks can be used for all types of tissue staining techniques, including immunohistochemistry and in situ hybridization.  In order to create a TMA block, cylindrical tissue samples are collected […]

Mohs IHC Staining Procedures: Why the ‘Kit’ Approach May Not Be the Best Mohs surgery is a quick and highly effective procedure designed to treat skin cancer. This surgical procedure can be used to treat basal cell carcinoma, squamous cell carcinoma, and melanoma and has success rates as high as 99 percent.1 During a Mohs […]

Chromogenic multiplexing procedures represent a set of techniques that permit the identification of two or more target antigens that can be microscopically differentiated by cellular location and/or color. Multiplexing has become increasingly popular as pathologists and other scientists have gained an appreciation for the significant clinical and technical benefits that such procedures provide.

Chromogenic multiplexing procedures represent a set of techniques that permit the identification of two or more target antigens that can be microscopically differentiated by cellular location and/or color. Multiplexing has become increasingly popular as pathologists and other scientists have gained an appreciation for the significant clinical and technical benefits that such procedures provide. Creating a […]