Chromogens

Betazoid DAB is the third-generation DAB product developed by Biocare. It is superior to conventional DAB and Cardassian DAB in terms of stability and staining intensity. This chromogen is compatible with horseradish peroxidase (HRP), not soluble in alcohol or xylene, and can be coverslipped just like any other DAB. When in the presence of peroxidase enzyme, Betazoid produces a brown stain. Betazoid DAB may increase antibody titers by two fold; and can be used manually or on automated stainers.

A permanent mounting system for HRP versus aqueous mounting, Romulin AEC produces a brick-red stain and is not soluble in alcohol, xylene or xylene substitutes. This system consists of four components: buffer, stabilizer, chromogen and hydrogen peroxide. This system is compatible with both manual and automatic coverslippers.

This is a permanent chromogen substrate kit that is compatible with horseradish peroxidase (HRP). It is not soluble in alcohol or xylene, and can be coverslipped just like DAB. When in the presence of a peroxidase enzyme, Bajoran purple produces a permanent lavender-purple stain. This product comes in a four-component system consisting of a ready-to-use buffer, stabilizer, chromogen and hydrogen peroxide. Bajoran Purple may also be used in double- and triple-stain procedures, nitrocellulose blots, and can be viewed by both brightfield and darkfield microscopy.

Vina Green is a novel permanent chromogen that produces a green stain in the presence of horseradish peroxidase (HRP). The kit consists of liquid Vina Green chromogen and buffer that are stable at least 4 hours at room temperature. Vina Green is clearly distinguishable from Warp Red and DAB on a single slide, enabling high flexibility for its application in Multiplex IHC. Developed for both manual and automated platforms, Vina Green is suitable for both immunohistochemistry (IHC) and in situ hybridization (ISH) applications including HPV, CMV, EBV, p63, TTF-1, Ki-67 and other targets antigens such as blood and lymphatic vessels, and basal and myoepithelial cells.

Deep Space Black™ is a novel permanent chromogen that produces a dark grey to black stain in the presence of horseradish peroxidase (HRP). The kit consists of liquid Deep Space Black chromogen and buffer that is stable for 8 hours at room temperature once mixed. Deep Space Black is clearly distinguishable from Warp Red™, DAB, Vina Green™ and Ferangi Blue™ on a single slide, enabling high flexibility for Multiplex IHC™ applications. Developed for both manual and automated platforms, Deep Space Black is suitable for both immunohistochemistry (IHC) and in situ hybridization (ISH) applications including HPV, CMV, EBV, Kappa, Lambda, p63, HMWCK, TTF-1, Ki-67, and other targets/antigens such as cytokeratins, melanoma, and basal and myoepithelial cells.

Biocare Medical’s Warp Red is a faster, sharper and more stable red chromogen. The newly formulated Warp Red is quick and sensitive, similar to DAB, providing an accelerated protocol and consistent staining quality. The sharp and intense red stain creates superior contrast for Multiplex IHC™. These advanced features of Warp Red result in a faster turnaround time, greater staining consistency and improved flexibility.

Vulcan Fast Red is insoluble in organic solvents and can be coverslipped with a permanent mounting media. This chromogen produces a bright fuchsin-red precipitate in the presence of alkaline phosphatase (AP) enzyme. For optimum results, use Immunocare TBS Wash Buffer. Not for use with PBS, as PBS is a competitive inhibitor of alkaline phosphatase and will dramatically reduce staining. Vulcan Fast Red can be viewed by both brightfield and darkfield microscopy.

Ferangi Blue Chromogen Kit

Ferangi Blue can be coverslipped with a non xylene- or toluene-based permanent mounting media. This chromogen produces a bright royal blue precipitate in the presence of alkaline phosphatase enzyme. For optimum results, use Immunocare TBS Wash Buffer. Not for use with PBS.