Fast Red Chromogen Kit consists of two solutions for the staining of formalin-fixed, paraffin-embedded (FFPE) tissues, as part of an immunohistochemistry (IHC) procedure using an alkaline phosphatase (AP) detection system, on Biocare Medical’s ONCORE Pro Automated Slide Stainer. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.
Immunohistochemistry (IHC) permits the visual identification of specific protein antigens in tissues for diagnostic purposes. Following application of the primary antibody, the presence of a target antigen is visualized by the sequential application of an enzyme-antibody conjugate that binds the primary antibody, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy. Fast Red is a widely used chromogen for immunohistochemical staining with alkaline phosphatase (AP) detection systems. In the presence of alkaline phosphatase enzyme, Fast Red produces a red precipitate. Fast Red Chromogen Kit contains two solutions: Fast Red chromogen and the corresponding buffer. It is intended for use with an AP detection system in an IHC staining procedure on the ONCORE Pro Automated Slide Stainer.