Deep Space BlackTM (DSB) is a novel permanent chromogen that produces a dark grey to black stain in the presence of horseradish peroxidase (HRP). The kit consists of liquid Deep Space Black Chromogen and Buffer that are stable for 8 hours at room temperature once mixed. Deep Space BlackTM is clearly distinguishable from Warp RedTM, DAB, Vina GreenTM and Ferangi BlueTM on a single slide, enabling high flexibility for its application in Multiplex IHCTM and double stains. Deep Space Black is suitable for both immunohistochemistry (IHC) and in situ hybridization applications including HPV, CMV, EBV, Kappa, Lambda, p63, HMWCK, TTF-1, Ki-67, and other targets/antigens such as cytokeratins, melanoma, and basal and myoepithelial cells.
The reaction product is insoluble in alcohols and xylene and, therefore, a variety of counterstains and permanent mounting media can be used with this chromogen kit.