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Biocare’s PromARK™ series introduces Rabbit-on-Canine HRP-Polymer Detection. This detection is specially designed for using rabbit antibodies on canine and feline tissues and utilizes biotin-free horseradish peroxidase (HRP) polymer technology. The advantages of this polymer technology are increased sensitivity, reduction of IHC steps (no Avidin/Biotin block or Link/Probe), and minimal cross-reactivity to endogenous canine and feline IgG. The Rabbit-on-Canine detection can be used with paraffin-embedded tissues and can be performed manually or on automated staining platforms. The addition of proprietary blockers to the detection reagent permits the use of any of Biocare Medical’s retrieval solutions (Reveal/Borg/Diva) or enzymes with the elimination of endogenous IgG or non-specific background staining. Temperature dependent protocols can be conveniently performed using Biocare’s Decloaking Chamber. The Rabbit-on-Canine HRP-Polymer may be combined with the Mouse-on-Canine HRP-Polymer to create a universal polymer detection that will label both mouse and rabbit antibodies with HRP. Refer to Technical Note #4 for additional information. The Rabbit-on-Canine HRP-Polymer may be combined with the Mouse-on-Canine AP- Polymer to create a double stain polymer detection that will label the rabbit antibody with HRP and the mouse antibody with AP. Refer to Technical Notes #5 and #6 for additional information.