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Denaturing Solution is used as an elution step when using a double stain technique (Biocare’s 7-step double stain procedure). This will ensure that the second staining protocol will not cross react with the first staining protocol. Solution A (yellow) is a concentrate that must be diluted with Solution B (red, dilution buffer) for proper elution. It is recommended that the Denaturing Solution be used at different dilutions depending on tissue fixation and the type of antigen retrieval method used.