Biocare’s PromARKTM series introduces Rabbit-on-Farma HRP-Polymer detection. The Rabbit-on-Farma HRP-Polymer is formulated to detect rabbit polyclonal or monoclonal antibodies and is affinity purified for minimal cross-reactivity to cow, horse, pig and sheep IgG proteins. The advantages of this polymer technology are increased sensitivity, reduction of IHC steps (no avidin/biotin block or link/enzyme conjugates), minimal cross-reactivity to endogenous IgG that allows multiple species flexibility, and in most cases, tissues do not require a protein block. The Rabbit-on-Farma detection can be used on paraffin-embedded tissues and can be performed manually or on automated staining platforms. This detection may be used with any of Biocare Medical’s retrieval solutions (Reveal/Borg/Diva) or digestive enzymes. Temperature dependent protocols can be conveniently performed using Biocare’s Decloaking Chamber. The Rabbit-on-Farma HRP-Polymer may be combined with the Mouse-on-Farma HRP-Polymer to prepare a universal HRP-Polymer detection that will label both mouse and rabbit antibodies with HRP. Refer to Technical Note #4 for additional information. The Rabbit-on-Farma HRP-Polymer may be combined with the Mouse-on-Farma AP-Polymer to prepare a double stain polymer detection that will label the rabbit antibody with HRP and the mouse antibody with AP. Refer to Technical Note #5 for additional information.
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