RET (10q11.21) Break Apart Orange/Green

Product Description

The RET (10q11.21) Break Apart FISH probe is a dual color probe, designed to detect chromosomal rearrangements involving the RET proto-oncogene (10q11.21). The RET proto-oncogene encodes a tyrosine kinase receptor and under normal conditions, it functions as a signaling molecule involved in cellular growth and differentiation. Chromosomal rearrangements involving RET were initially identified in papillary thyroid carcinoma (1). However, RET gene rearrangements have been identified in Non-Small-Cell Lung Cancer (NSCLC) patients (2). RET gene rearrangements in NSCLC result in a novel gene fusion event involving RET and KIF5B gene (3). This genetic aberration is believed to play a role in oncogenesis in NSCLC (3). RET gene rearrangements can be identified using Fluorescence in situ Hybridization (FISH).

Specifications

1. Tsuta, K., T. Kohno, A. Yoshida, Y. Shimada, H. Asamura, K. Furuta, and R. Kushima. “RET-rearranged Non-small-cell Lung Carcinoma: A Clinicopathological and Molecular Analysis.” British Journal of Cancer 110 (2014): 1571-578.
2. Bos, Marc, Masyar Gardizi, Hans- Ulrich Schildhaus, Reinhard Buettner, and Juergen Wolf. “Activate RET and ROS: Two New Driver Mutations in Lung Adenocarcinoma.” Translational Lung Cancer Research 2.2 (2013): 112-21.
3. Ju, Y. S., W.-C. Lee, J.-Y. Shin, S. Lee, T. Bleazard, J.-K. Won, Y. T. Kim, J.-I. Kim, J.-H. Kang, and J.-S. Seo. “A Transforming KIF5B and RET Gene Fusion in Lung Adenocarcinoma Revealed from Wholegenome and Transcriptome Sequencing.” Genome Research (2012): 436-45.
4. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.