p63 + P504S (PIN)

Product Description

p63, a homolog of the tumor suppressor p53, has been identified in proliferating basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium and prostate.¹ p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate.² P504S, also known as α-methylacyl coenzyme A racemase (AMACR), is a peroxisomal and mitochondrial enzyme that plays a role in bile acid synthesis and β-oxidation of branched chain fatty acids.³ P504S was initially identified from a cDNA library as a gene that is overexpressed in human prostate cancer; with little or no expression in normal prostate. ^4,5

Specifications

1. Yang A, et. al. p63, a p53 Homolog at 3q27–29, Encodes Multiple Products with Transactivating, Death-Inducing, and Dominant-Negative Activities. Mol Cell. 1998;2:305-16.
2. Signoretti S, et. al. p63 Is a Prostate Basal Cell Marker and Is Required for Prostate Development. Am J Pathol. 2000;157:1769-75.
3. Ferdinandusse S, et. al. Subcellular localization and physiological role of α- methylacyl-CoA racemase. J Lipid Res. 2000; 41:1890-6.
4. Xu J, et. al. Identification of Differentially Expressed Genes in Human Prostate Cancer Using Subtraction and Microarray. Cancer Res. 2000; 60:1677-82.
5. Rubin MA, et. al. α-Methylacyl Coenzyme A Racemase as a Tissue Biomarker for Prostate Cancer. JAMA. 2002; 287:1662-70.
6. Luo J, et. al. Alpha-methylacyl-CoA racemase: a new molecular marker for prostate cancer. Cancer Res. 2002; 62:2220-6.
7. Zhou M, et. al. Alpha-Methylacyl-CoA Racemase A Novel Tumor Marker Overexpressed in Several Human Cancers and Their Precursor Lesions. Am J Surg Pathol. 2002; 26:926-31.
8. Wu CL, et. al. Analysis of α-Methylacyl-CoA Racemase (P504S) Expression in High-Grade Prostatic Intraepithelial Neoplasia. Hum Pathol. 2004; 35:1008-13.
9. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
10. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved guideline-Third Edition CLSI document M29-A3 Wayne, PA 2005.