The conjugated goat anti-mouse polymer alkaline phosphatase (AP) and the conjugated goat anti-rabbit polymer horseradish peroxidase (HRP) secondary antibodies react with both heavy and light chains on mouse and rabbit IgG respectively. This innovative AP- and HRP-polymerization technology provides a significant increase in staining sensitivity when compared to other conventional AP- or HRP-conjugated secondary antibodies. Avidin-biotin blocking procedures are not necessary when using the MACH 2 conjugated secondary antibodies. It is specially designed for a rapid double stain procedure. An antibody cocktail with a rabbit monoclonal/polyclonal and a mouse monoclonal antibody must be used. The overall staining procedure can be done in 4 steps and in less than two hours. It can be used manually or used with automated stainers.