Cytokeratin 5 (CK5)

Product Description

The Cytokeratin 5 (CK5) (R) [EP42] antibody is a rabbit monoclonal antibody raised for the detection of cytokeratin 5, a type II intermediate filament protein. CK5 is expressed in the active basal layers of most stratified squamous epithelia.¹⁵ CK5 has been identified as a specific immunohistochemical marker for lung squamous cell carcinoma (SqCC), with minimal expression in lung adenocarcinoma.^16,17 In a published study comparing rabbit monoclonal CK5 to mouse monoclonal CK5/6, CK5 demonstrated 84% sensitivity and 100% specificity for lung SqCC, whereas CK5/6 showed 80% sensitivity and 97% specificity.^16,17 These findings support CK5 as a potentially more specific marker for lung SqCC, as CK6 mRNA has been detected in lung adenocarcinomas. The CK5 antibody has been optimized for use in the detection of lung squamous cell carcinoma. Other tumor types have not been evaluated. CK5 is also distributed in various non-keratinizing stratified squamous epithelia, including the mucosa of the tongue, basal epithelial layers, hair follicles, and trachea. Expression is also observed in basal cells of the prostate gland and myoepithelial cells of mammary glands.18 Additionally, CK5 is expressed in most epithelial and biphasic mesotheliomas.¹⁸

Specifications

1. Mukhopadhyay S, Katzenstein AL. Subclassification of non-small cell lung carcinomas lacking morphologic differentiation on biopsy specimens: Utility of an immunohistochemical panel containing TTF-1, Napsin A, p63, and CK5/6. Am J Surg Pathol. 2011 Jan; 35(1):15-25.
2. Tacha D, Yu C, Haas T. TTF-1, Napsin A, p63, TRIM29, Desmoglein-3 and CK5: An Evaluation of Sensitivity and Specificity and Correlation of Tumor Grade for Lung Squamous Cell Carcinoma vs. Lung Adenocarcinoma. Modern Pathology; USCAP, 2011 (Abstract Accepted)
3. Tacha D, Zhou D, Henshall-Powell RL¹. Distinguishing Adenocarcinoma from Squamous Cell Carcinoma in Lung Using Double Stains p63+ CK5 and TTF-1 + Napsin A. Modern Pathology; Pathology Volume 23, Supplement 1, Feb 2010; Abstract 1852, page 222A.
4. Terry J, et al. Optimal immunohistochemical markers for distinguishing lung adenocarcinomas from squamous cell carcinomas in small tumor samples. Am J Surg Pathol. 2010 Dec; 34(12):1805-11.
5. Kargi A, Gurel D, Tuna B. The diagnostic value of TTF-1, CK 5/6, and p63 immunostaining in classification of lung carcinomas. Appl Immunohistochem Mol Morphol. 2007 Dec; 15(4):415-20.
6. Miettinen M, Sarlomo-Rikala M. Expression of calretinin, thrombomodulin, keratin
5, and mesothelin in lung carcinomas of different types: an immunohistochemical analysis of 596 tumors in comparison with epithelioid mesotheliomas of the pleura. Am J Surg Pathol. 2003 Feb; 27(2):150-8.
7. Bocker W, et al. Common adult stem cells in the human breast give rise to glandular and myoepithelial cell lineages: a new cell biological concept. Lab Invest. 2002 Jun; 82 (6):737-46.
8. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
9. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved guideline-Third Edition CLSI document M29-A3 Wayne, PA 2005