CD3 is expressed throughout the T-cell differentiation process. CD3 is a highly specific and sensitive T-cell lineage marker, making it ideal for the immunohistochemical analysis of lymphohematologic malignancies. Notable exceptions include some of the more aggressive large T-cell lymphomas and CD30 (Ki-1) positive anaplastic large cell lymphomas, which may not express detectable antigen (1-3). A monoclonal antibody to human CD3 is regarded as a reliable pan T-cell antibody used in identifying lymphomas and lymphocytic colitis in paraffin sections (4).
1. Campana D, et al. The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage. J Immunol. 1987 Jan; 138(2):648-55.
2. Cabeçadas JM, Isaacson PG. Phenotyping of T-cell lymphomas in paraffin sectionswhich antibodies? Histopathology. 1991 Nov; 19(5):419-24.
3. Steward M, et al. Production and characterization of a new monoclonal antibody effective in recognizing the CD3 T-cell associated antigen in formalin-fixed embedded tissue. Histopathology. 1997 Jan; 30(1):16-22.
4. “CD3 Assessment Run 37 2013.” NordiQC. NordiQC, 04 Dec. 2013. Web. 16 June 2015.
5. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
6. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.