Buff Up on Buffers and Surfactants

Wash buffers are an integral part of immunohistochemical (IHC) procedures. They are used after each incubation step to wash away excess reagent from the specimen and maintain a constant pH by “soaking up” any free hydrogen (H+) ions. This feature helps maintain the morphological characteristics of antibodies and their epitopes, which enables specific binding. The […]

Biocare Basics: Optimization

Proper optimization is vital for successful immunohistochemical (IHC) testing. An optimal IHC protocol can be characterized by its ability to consistently and unambiguously determine how the antigen is expressed in the tissue. Optimization is fundamental to reducing the risk of both false positive and false negative results.1 The protocol must be able to identify both […]

Alternatives to Lysol for Cleaning Automated Instrumentation

Regular cleaning is an essential part of instrument maintenance, but what happens if you don’t have Lysol? Following recommended cleaning procedures to remove residues and sterilize instrument components will improve instrument performance and help extend the instrument’s lifespan. This white paper looks at Lysol alternatives for cleaning to ensure your automation platform keeps running perfectly! […]

Biocare Basics: Validation

Validation is an essential step for setting up a new laboratory procedure, whether it incorporates immunohistochemical (IHC) or routine ematoxylin and eosin (H&E) staining. The validation process involves repeatedly testing the procedure over a large quantity and range of specimens in order to ensure the procedure will yield consistent and reproducible results. This, in turn, […]

The Benefits of Using Tissue Microarrays (TMAs) In Your Lab

Tissue microarrays (TMAs) are paraffin blocks that include multiple samples from different tissue types meant to provide a comprehensive multiplex staining profile. The sections from TMA blocks can be used for all types of tissue staining techniques, including immunohistochemistry and in situ hybridization.  In order to create a TMA block, cylindrical tissue samples are collected […]

Performing IHC Staining Procedures in the Mohs Surgery Lab: Why the ‘Kit’ Approach May Not Be the Best Approach

Mohs IHC Staining Procedures: Why the ‘Kit’ Approach May Not Be the Best Mohs surgery is a quick and highly effective procedure designed to treat skin cancer. This surgical procedure can be used to treat basal cell carcinoma, squamous cell carcinoma, and melanoma and has success rates as high as 99 percent.1 During a Mohs […]

Performing Simultaneous Chromogenic IHC Multiplexing Procedures

Chromogenic multiplexing procedures represent a set of techniques that permit the identification of two or more target antigens that can be microscopically differentiated by cellular location and/or color. Multiplexing has become increasingly popular as pathologists and other scientists have gained an appreciation for the significant clinical and technical benefits that such procedures provide.

Creating Sequential Chromogenic IHC Multiplexing Procedures…

Chromogenic multiplexing procedures represent a set of techniques that permit the identification of two or more target antigens that can be microscopically differentiated by cellular location and/or color. Multiplexing has become increasingly popular as pathologists and other scientists have gained an appreciation for the significant clinical and technical benefits that such procedures provide. Creating a […]

CellPath Signs Exclusive Agreement with Biocare Medical to Become Sole UK Distributor

Biocare Medical and CellPath are pleased to announce our newest exclusive partnership! From 1st June 2021, CellPath will become Biocare Medical’s sole UK distributor, supplying world-class automated immunohistochemistry instrumentation and the full range of reagents for IHC, ISH, and FISHlab testing. Biocare Medical is an innovator in development, manufacture, and supply, becoming the market leader […]

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