Antigen Retrieval Terms for IHC
Antigen retrieval (AR) is a fundamental part of any IHC protocol. However, with so many different names for this one process, it can be difficult to recognize what an individual protocol or paper is referring to. Why is it sometimes called “retrieval” and other times simply “pretreatment?” Is ER the same as AR? What is PIER and how does it compare to HIER? To get to the bottom of these questions, we must take a look at the purpose of antigen retrieval and which techniques for antigen retrieval are in use today.
Learn How Heat-Induced Retrieval and Enzymatic Retrieval Differ