NeoPATH Pro Antigen Retrieval 1 (AR1)

Product Description

The NeoPATH Pro Antigen Retrieval 1 (AR1) is designed as a Tris-EDTA-based buffer used in heat-induced antigen retrieval (HIER) of formalin-fixed, paraffin-embedded (FFPE) tissues. HIER is the process of heating the slide-mounted specimen material in an antigen retrieval solution, followed by a cooling-off period. Retrieval solutions with paraffin emulsifiers (surfactants) followed by a rinsing reagent can deparaffinize and retrieve tissues, thus eliminating the deparaffinization step with xylenes and alcohols.

Most immunohistochemistry (IHC) specimens are routinely fixed in formalin and, while formalin provides excellent preservation of morphology, it can greatly diminish the sensitivity to IHC. Formalin creates cross-links that alter protein biochemistry and can block antigenic sites. Antigen retrieval is needed to break these cross-links and expose the antigen’s epitope. Biocare’s Antigen retrieval reagents are used in heat-induced antigen retrieval (HIER) of formalin-fixed, paraffinembedded (FFPE) tissues. The antigen retrieval process has many variables, including the target antigen, the antibody, fixation time, and tissue processing as well as the antigen retrieval method. For the HIER method, which is the process of heating the slide-mounted specimen material in an antigen retrieval solution, followed by a cooling-off period, the pH and composition of the retrieval solution are important factors.

NeoPATH Pro Antigen Retrieval 1 (AR1) is capable of partially breaking the covalent bonds of formalin-fixed tissue, as part of an IHC staining procedure on the NeoPATH Pro Automated Slide Stainer. Pretreatment of FFPE tissues with NeoPATH Pro Antigen Retrieval 1 (AR1) can significantly increase staining intensity. The solution is provided ready-to-use and is intended to be applied as defined by the staining protocols on the NeoPATH Pro Automated Slide Stainer.

Specifications