Decloaking Chamber™

Decloaking Chamber™ Plus

Decloaking Chamber™ PlusThe Decloaking Chamber™ Plus offers a combination of complete walk-away and quality control capabilities, ensuring optimal antigen retrieval with superior and consistent results. This system features a cooling fan for more rapid antigen retrieval, producing results in as little as 35 minutes. An optional stainless steel rack holder allows easy removal of plastic slide containers. Furthermore, a USB port enables the laboratory to utilize a flash drive to track and save run data for quality control purposes. The pressure gauge has a new feature in which the needle remains at the highest pressure reading during the run, validating that antigen retrieval has occurred properly. When the procedure is complete, an audible beep alerts the user. The Decloaking Chamber™ Plus, with up to 96-slide capacity, is well suited for high-throughput antigen retrieval. (Cat. No. DC2008)

Standardized Results

  • Crisp and even staining
  • Very gentle on tissues
  • Reproducible results
  • Reduction of false negatives

A Complete Quality Control System

  • HIER buffer, pH indicators change color with temperature
  • Two-step deparaffinization and retrieval buffers
  • Steam monitor strips for temperature verification
  • Audible alert upon run completion

Decloaking Chamber™

Decloaking Chamber™A Digital Programmable Pressure System for HIER. Specifically for heat-induced epitope retrieval, the Decloaking Chamber Pro is an excellent tool for optimized, consistent immunohistochemistry staining, in conjunction with the appropriate buffer solution. (Cat. No. DC2002)

CE Listed, ETL approved, U.S. patent number 6,580,056, June 17, 2003

Ancillaries

Accessories Cat. No. Volume Download
Kling-On Microscope Slides SFH1103 A, B 1 gross (144 slides), 10 gross (1 case) Data Sheet
Tissue Tek Slide Holders and Containers TTSET-4PK 4-pack N/A
Decloaker Gasket RMDC037 1 each N/A
Coplin Jar CJ-3PK 3-pack N/A

Microtiter Plate Platform™

The Microtiter Plate Platform facilitates antigen retrieval for free floating sections in preparation for IHC or IF and is particularly useful for whole brain sections. The platform holds microtiter plates containing tissues away from the source of heat during antigen retrieval in an HIER device. This allows for more even, standardized results for immunohistochemistry and immunofluorescence (IF) staining techniques.

Standard Microtiter Plates holding free-floating sections are filled with a retrieval solution, placed on the Microtiter Plate Platform, and lowered into the Decloaking Chamber Pro. This is especially critical when using thick sections of delicate tissue such as brain tissues. The Microtiter Plate Platform also stabilizes the specimens and enables ease of use. It has been rigorously tested for heat stability and is ISO compliant.

Microtiter Plate Platform Cat. No. Volume
Microtiter Plate Platform MPP001 1 ea.

Steam Monitor Strips for Quality Control

The steam monitor strips are heat and pressure sensitive, acting as a quality control check and confirms that the chamber has reached full temperature and pressure as evidenced by the dark brown to black color. The strips can be placed in your laboratory notebook as a permanent record for that run.

pH Strips for Quality Control
The pH of citrate-based retrieval solutions increase as the temperature rises. To assure quality control and consistent IHC staining, Biocare has developed heat-tested pH strips that give accurate and the true pH at high temperatures. The pH of a solution should be recorded at the temperature used. Using these pH strips, the laboratory investigator can now record pH on a daily basis, and comply with CAP and JCOH inspection.

Quality Control Strips Cat. No. Volume Download
Steam Monitor Strips 613 H, C, D 25, 100, 250 Strips Data Sheet
QC pH Strips pH 4-7 pH615 100-pack N/A
QC pH Strips pH 7.5-10.5 pH616 100-pack N/A

Decloaking Chamber™ Plus System Specifications

Specifications Capacities
Size 12″ (W) x 11″ (D) x 11″ (H) / 31cm (W) x 29cm (D) x 28cm (H)
Weight 8.2 lbs, 3.7 kg
Temperature Range 37°C to 125°C (± 5°C)
Pan Capacity 3.2 liters (maximum)
Slide capacity Up to 96 slides (in plastic Tissue Tek containers)
Up to 60 slides (in plastic Coplin jars)

Decloaking Chamber ™ System Specifications

Specifications Capacities
Size 13” (W) x 12” (D) x 12.5” (H) / 33 cm (W) x 30 cm (D) x 32 cm (H)
Weight 10.5 lbs. 4.8 kg
Temperature Range 37 °C to 125 °C (+/- 5 °C)
Pan Capacity 3.2 liters (maximum)

Ordering Information

Decloaking Chamber™ Plus (United States) Description Cat. No. Volume
Decloaking Chamber™ Plus With Rack Holder; 110-120V, 800W, 60Hz DC2008US-RH 1 ea.
Decloaking Chamber™ Plus Without Rack Holder; 110-120V, 800W, 60Hz DC2008US 1 ea.
Decloaking Chamber™ Plus (International) Description Cat. No. Volume
Decloaking Chamber™ Plus With Rack Holder; 220-240V, 900W, 50Hz DC2008INTL-RH 1 ea.
Decloaking Chamber™ Plus Without Rack Holder; 220-240V, 900W, 50Hz DC2008INTL 1 ea.
Decloaking Chamber™ Description Cat. No. Volume
Decloaking Chamber™ 115V, 800W, 60Hz, for use within the United States DC2002-CE 1 ea.
Decloaking Chamber™ 230V, 800W, 50Hz, international distribution (Non-CE approved) DC2002-220 1 ea.