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Nuclear Decloaker is a Tris buffer heat retrieval solution, pH 9.5. It is specially designed for nuclear antigens including ER, PR, Ki-67, p53, cyclin D1, TdT and TTF-1. Other antibodies that show improved staining include ALK1, ALKc, bcl-2, CA125, CD3 (M), CD15, CD30, CD31, CD79a, MART-1, Tyrosinase and Villin. Nuclear Decloaker can be used with Biocare’s digital electric pressure cooker (Decloaking Chamber), a steamer, a water bath, or a microwave oven. Nuclear Decloaker incorporates Assure™ technology, a color-coded high temperature pH indicator solution. The end-user is assured by visual inspection that the solution is at the correct dilution and pH. This product is specially formulated for superior pH stability at high temperatures and will help prevent the possibility of losing pH sensitive antigens. Nuclear Decloaker is non-toxic, non-flammable, odorless and sodium azide and thimerosal free.