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Pronase (Streptomyces griseus) is a commonly used digestive enzyme. In formalin-fixed paraffin-embedded tissues, certain antibody protocols require enzyme pretreatment for proper immunohistochemical staining. CAREZYME III is an aggressive enzyme and can be used at room temperature or at 37°C. Certain antibodies such as EGFR require this type of aggressive enzyme digestion. This product is provided as a two component system and can be used at different concentrations. CAREZYME III is not compatible with antibodies AE1/AE3 or AE3. It can also be used on an automated staining system.