SSC Wash Buffer is a ready-to-use saline sodium citrate buffer (pH 7.0-7.5) that contains 0.3% NP40 and Proclin 950 as a preservative. SSC Wash Buffer contains 30 mM citrate, which is commonly identified as 2X SSC. This buffer may be used as a post-hybridization wash buffer for in situ hybridization procedures.
1. Wilkinson DG. In Situ Hybridization: A Practical Approach (Practical Approach Series). 2nd Ed. Oxford: Oxford University Press, 1999.
2. Nuovo GJ. In Situ Molecular Pathology and Co-Expression Analyses. 1st Ed. San Diego: Academic Press, 2013.
3. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.