PTEN del-TECT™ Four Color

Product Description

The PTEN del-TECT (4 Color) FISH probe greatly increases the reliability of deletion detection, significantly decreasing the number of falsepositive events2 caused by cell truncation, an artifact of FFPE tissue processing. The PTEN Test probe is labeled in Orange and detects deletions of the PTEN gene. Probe A (WAPAL/BMPR1A) is located centromeric to the test probe and is labeled in green. Probe B (FAS) is located telomeric to the test probe and is labeled in aqua. The combination of the three colored probes (green, orange and aqua) in close proximity assists not only in eliminating the possibility of truncation of this region, but also in determining the actual size of the deletion. The centromere 10 probe labeled in red is included to help determine if chromosome 10 monosomies or polysomies are present.

Specifications

1. Yoshimoto M, Cutz J-C, Nuin PAS, Joshua AM, Bayani J, Evans AJ, Zielenska M,Squire JA. Interphase FISH Analysis of PTEN in Histologic Sections Shows Genomic Deletions are Present in 68% of Primary Prostate Cancer and 23% of High-Grade Prostatic Intra-Epithelial Neoplasia. Cancer Genetics and Cytogenetics 169:128-37, 2006.
2. Yoshimoto M, Cunha IW, Coudry RA, Fonseca FP, Torres CH, Soares FA, Squire JA. FISH analysis of 107 prostate cancers shows that PTEN genomic deletion is associated with poor clinical outcome. British Journal of Cancer 97(5):678-85, 2007.
3. Yoshimoto M, Cunha IW, Coudry RA, Joshua A, FP Fonseca, Zielenska M, Soares FA, Squire JA. Absence of TMPRSS2:ERG fusions and PTEN losses identifies prostate cancer genomic grade with favorable outcome. Modern Pathology Epub ahead of print, 2008.
4. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.