ONCORE Pro FISH Kit

Product Description

ONCORE Pro FISH Kit contains reagents used in the pretreatment of formalin-fixed paraffin-embedded tissues (FFPE) to enhance probe accessibility to nucleic acid targets as well as a post-hybridization buffer used to eliminate non-specific binding of the probe. In FFPE tissues, FISH protocols usually require a combination of acid, heat, and enzymatic pretreatment to achieve proper staining. Hydrochloric acid (HCl, 0.2N) is a weak acid commonly used in FISH protocols. FISH Retrieval is a citrate-based heat pretreatment solution atpH 6.0. It is a non-toxic, non-flammable and odorless solution that contains sodium azide while being thimerosal-free. FISHzyme is a commonly used pepsin digestive enzyme. Combination of all 3 pretreatment reagents provides a synergistic effect on probe accessibility to nucleic acid targets. Reagent Alcohol, 70% is necessary for tissue dehydration before a probe application. PF SSC Wash Buffer is a ready-to-use saline sodium citrate buffer (pH 7.0-7.5) that contains 0.3% NP40 and Proclin 950 as a preservative.

PF SSC Wash Buffer contains 30 mM citrate, which is commonly identified as 2X SSC. This buffer is used as a post-hybridization wash buffer in FISH procedures.

Specifications

1. Occupational Safety and Health Standards: Occupational exposure to hazardous chemicals in laboratories. (29 CFR Part 1910.1450). Fed. Register.
2. Directive 2000/54/EC of the European Parliament and Council of 18 September 2000 on the protection of workers from risks related to exposure to biological agents at work.
3. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.