Multiplex Detection 2 is a cocktail of HRP anti-mouse antibody conjugate and AP anti-rabbit antibody conjugate suitable for the detection of mouse and rabbit primary antibodies, respectively, as part of an IHC double-stain procedure on the ONCORE Automated Staining Instrument. Multiplex Detection 2 is intended to be used in combination with DAB Chromogen Kit (ORI6011K) to produce a brown stain at the site of mouse primary antibodies, and Fast Red Chromogen Kit (ORI6042K) to produce a red stain at the site of rabbit primary antibodies.
Multiplex Detection 2 is prepared using a proprietary micropolymer technology that provides a highly sensitive detection system, with minimal background staining. Multiplex Detection 2 does not use biotin or streptavidin, thus avoiding non-specific staining associated with endogenous biotin. Multiplex Detection 2 is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE instrument.