Multiplex Detection 1 is a cocktail of alkaline phosphatase (AP) antimouse antibody conjugate and horseradish peroxidase (HRP) anti-rabbit antibody conjugate intended for use in the detection of mouse IgG and IgM and rabbit IgG primary antibodies on formalin-fixed, paraffin embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure performed on Biocare Medical’s ONCORE Pro Automated Staining Instrument and visualized by light microscopy. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.
Immunohistochemistry (IHC) permits the visual identification of specific protein antigens in tissues for diagnostic purposes. Following application of the primary antibody, the presence of a target antigen is visualized by the sequential application of an enzyme-antibody conjugate that binds the primary antibody, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy. Multiplex Detection 1 is a cocktail of AP anti-mouse antibody conjugate and HRP anti-rabbit antibody conjugate suitable for the detection of mouse and rabbit primary antibodies, respectively, as part of an IHC double-stain procedure on the ONCORE Pro Automated Staining Instrument. Multiplex Detection