Multiplex Detection 2 is a cocktail of horseradish peroxidase (HRP) antimouse antibody conjugate and alkaline phosphatase (AP) anti-rabbit antibody conjugate intended for use in the detection of mouse IgG and IgM and rabbit IgG primary antibodies on formalin-fixed, paraffinembedded (FFPE) tissues in an immunohistochemistry (IHC) procedure performed on Biocare Medical’s ONCORE Pro Automated Staining Instrument and visualized by light microscopy. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.Immunohistochemistry (IHC) permits the visual identification of specific protein antigens in tissues for diagnostic purposes. Following application of the primary antibody, the presence of a target antigen is visualized by the sequential application of an enzyme-antibody conjugate that binds the primary antibody, and a chromogen reagent, to produce a colored reaction product that is visible by light microscopy. Multiplex Detection 2 is a cocktail of HRP anti-mouse antibody conjugate and AP anti-rabbit antibody conjugate suitable for the detection of mouse and rabbit primary antibodies, respectively, as part of an IHC double-stain procedure on the ONCORE Pro Automated Staining Instrument. Multiplex Detection 2 is intended to be used in combination with ONCORE Pro DAB Chromogen (OPRI6056K) to produce a brown stain at the site of mouse primary antibodies, and ONCORE Pro Fast Red Chromogen (OPRI6042K) to produce a red stain at the site of rabbit primary antibodies. Multiplex Detection 2 is prepared using a proprietary micro-polymer technology that provides a highly sensitive detection system, with minimal background staining. Multiplex Detection 2 does not use biotin or streptavidin, thus avoiding non-specific staining associated with endogenous biotin. Multiplex Detection 2 is provided ready-to-use and is intended to be applied as defined by the staining protocols on the ONCORE Pro instrument.