E-Cadherin is a transmembrane glycoprotein that plays a key role in cellcell adhesion in epithelial tissues (1-2). The adherens junction between epithelial cells is comprised of extracellular domains of E-Cadherin from adjacent cells, which interact through a molecular zipper motif. In normal tissues, immunostaining of E-Cadherin is localized to the membrane of epithelial cells, consistent with its role in cell adhesion. Immunohistochemical studies have shown E-Cadherin to be expressed in breast ductal carcinoma with loss of expression in lobular carcinoma (1-2). As a result, mouse monoclonal anti-E-Cadherin [HECD-1 has been used by pathologists to differentiate between ductal and lobular carcinomas of the breast, with currently published sensitivity and specificity of approximately 90% (3). Rabbit monoclonal E-Cadherin antibody may combine the best properties of both monoclonal antibodies and rabbit antisera.
1. de Deus Moura R, et al. Immunohistochemistry applied to the differential diagnosis between ductal and lobular carcinoma of the breast. Appl Immuohistochem Mol Morphol. 2013 Jan;21(1):1-12.
2. Dabbs DJ, Bhargava R, Chivukula M. Lobular versus ductal breast neoplasms: the diagnostic utility of p120 catenin. Am J Surg Path. 2007 Mar;31(3):427-37.
3. Moriya T, et al. The role of immunohistochemistry in the differential diagnosis of breast lesions. Pathology. 2009 Jan;41(1):68-76.
4. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
5. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.