DNA Hybridization Buffer is a modified Denhardt’s solution in citrate buffer containing formamide and a mixture of blocking reagents. When used as a diluent for DNA targeting probes in an ISH procedure, DNA Hybridization Buffer facilitates spreading of probes and reduces drying of DNA during hybridization reactions. Effective hybridization may facilitate enhanced staining and reduce artifacts.
1. Wilkinson DG. In Situ Hybridization: A Practical Approach (Practical Approach Series). 2nd Ed. Oxford: Oxford University Press, 1999.
2. Nuovo GJ. In Situ Molecular Pathology and Co-Expression Analyses. 1st Ed. San Diego: Academic Press, 2013.
3. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
4. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory Workers from Occupationally Acquired Infections; Approved Guideline-Fourth Edition CLSI document M29-A4 Wayne, PA 2014.