Cytokeratin 5/14 Cocktail

Product Description

CK5 is distributed in many non-keratinizing stratified squamous epithelia such as tongue mucosa, basal epithelia, hair follicles, trachea, as well as basal cells in prostate glands and myoepithelial cells in mammary glands.^15-19 CK5 is also expressed in most epithelial and biphasic mesotheliomas. CK5 has also been noted in large cell carcinomas and pulmonary squamous cell carcinomas.¹⁸ CK5 is expressed in luminal Type B breast cancers (triple negative).^17,19

CK14 is a human intermediate filament protein of 50kDa. CK14 can be used to distinguish stratified epithelial cells from simple epithelial cells. It is expressed in basal epithelium in prostate and myoepithelium in normal breast.^15-19 CK14 is a useful marker in the differential diagnosis of squamous cell carcinoma with poor clinical outcome.¹⁸ CK14 is also expressed in luminal Type B breast cancers, similar to CK5. Cytokeratin 5/14 Cocktail may be used to identify basal cells in prostate and myoepithelium cells in breast cancer.^15-19

Specifications

1. Abrahams NA, et al. Validation of cytokeratin 5/6 as an effective substitute for keratin 903 in the differentiation of benign from malignant glands in prostate needle biopsies. Histopathology. 2002 Jul; 41(1):35-41.
2. Shah RB, et al. Comparison of the basal cell-specific markers, 34betaE12 and p63, in the diagnosis of prostate cancer. Am J Surg Pathol. 2002 Sep; 26(9):1161-8.
3. Bhargava R, et al. CK5 is more sensitive than CK5/6 in identifying the “basal-like” phenotype of breast carcinoma. Am J Clin Pathol. 2008 Nov; 130(5):724-30.
4. Reis-Filho JS, et al. Distribution of p63, cytokeratins 5/6 and cytokeratin 14 in 51 normal and 400 neoplastic human tissue samples using TARP-4 multi-tumor tissue microarray. Virchows Arch. 2003 Aug; 443(2):122-32.
5. Laakso M, et al. Cytokeratin 5/14-positive breast cancer: true basal phenotype confined to BRCA1 tumors. Mod Pathol. 2005 Oct; 18(10):1321-8.
6. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
7. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved guideline-Third Edition CLSI document M29-A3 Wayne, PA 2005