Cytokeratin 18 antibody (CK18) [DC10] is a 45 kDa acidic intermediate filament protein. It is normally co-expressed with Cytokeratin 8 and is found in most simple ductal and glandular epithelia. Studies have shown that this antibody reacts with a wide variety of simple epithelia such as gastrointestinal tract, lung, breast, pancreas, ovary and thyroid tumors, whereas tumor cells of non-epithelial origin such as glioma, melanoma and osteosarcoma are not reactive. It also does not react with stratified squamous epithelium on most squamous cell carcinoma.
1. Lauerova L, et al. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504.
2. Shao MM, et al. Keratin expression in breast cancers. Virchows Arch. 2012 Sept;461(3):313-22.
3. Fareed KR, et al. Caspase-cleaved cytokeratin-18 and tumor regression in gastrooesophageal adenocarcinomas treated with neoadjuvant chemotherapy. World J Gastroenterol. 2012 Apr 28;18(16):1915-20.
4. Young GD, et al. Differential expression and biodistribution of cytokeratin 18 and desmoplakins in non-small cell lung carcinoma subtypes. Lung Cancer. 2002 May;36 (2):133-41.
5. Woelfle U, et al. Down-regulated expression of cytokeratin 18 promotes progression of human breast cancer. Clin Cancer Res. 2004 Apr 15;10(8):2670-4.
6. Center for Disease Control Manual. Guide: Safety Management, NO. CDC-22, Atlanta, GA. April 30, 1976 “Decontamination of Laboratory Sink Drains to Remove Azide Salts.”
7. Clinical and Laboratory Standards Institute (CLSI). Protection of Laboratory workers from occupationally Acquired Infections; Approved guideline-Third Edition CLSI document M29-A3 Wayne, PA 2005.