Improved Accuracy of Non-Small Cell Lung Cancer Phenotyping through Uniplex Staining Technology

Uniplex IHC – Aiding in Non-Small Cell Lung Cancer Phenotyping

As new discoveries and innovations continue to shape the diagnostics
industry, the use of molecular testing to supplement IHC results has
become increasingly popular. This industry shift has created a greater
need to conserve tissue for additional testing, as is the case when
diagnosing non-small cell lung cancers. Non-small cell lung cancer
can be classified into several types. The two main types are Lung
Adenocarcinomas (LADC) and Squamous Cell Carcinomas (SqCC) and
can be differentiated by up to 6 distinct markers

Key Markers for LADC & SqCC

• p40
• p63
• TTF-1
• CK5
• Desmoglein 3
• Napsin A

Studies have shown that a panel of the DSG3, p40, and TTF-1 markers is the most specific and sensitive method to distinguish between the two.
However, utilizing multiple antibodies with a common species of origin creates problems for traditional simultaneous IHC multiplex.

To match the highest standards of accuracy while still conserving precious tissue, Biocare has developed an IHC assay utilizing a novel uniplex
cocktail of DSG3 + p40, stained with DAB, sequentially followed by TTF-1, stained with Fast Red. Designed for use on a single tissue section,
this method combines the tissue preservation of a multiplex procedure with the specificity and sensitivity of a full staining panel.

In a study of previously diagnosed archival whole tissue lung cancer cases, staining with Biocare’s DSG3 + p40 uniplex cocktail and TTF-1
resulted in 4% or 1 out of every 25 cases being reevaluated and/or reclassified under a different diagnosis.

With each cancer type having its own targeted therapy, even the smallest differences in testing sensitivity can lead to divergent treatment
plans with dramatically different outcomes. Biocare’s DSG3 + p40 and TTF-1 IHC assay greatly improves the accuracy of crucial lung tumor
phenotyping while conserving tissue for molecular or target therapeutic testing.

To learn more about how Biocare’s Uniplex cocktails may aid in helping improve the accuracy of non-small cell lung cancer testing, please call
800-799-9499 or go online:

Tacha, D. (2018, March). et al. Strategies of Uniplex and Multiplex IHC Staining in Limited Tissue Biopsies. Poster session presented at the annual convention of the United States and Canadian Academy of Pathology, Vancouver, BC, Canada.