Product Description
Fast Red is a widely used chromogen for immunohistochemical staining. When in the presence of alkaline phosphatase (AP) enzyme, Vulcan Fast Red produces a bright fuchsin-red precipitate that is insoluble in organic solvents and can be coverslipped with a permanent mounting media. This product is available in a stable two-component system, consisting of Vulcan Fast Red Chromogen and Vulcan Buffer. When using an alkaline phosphatase system, tris buffer (pH 7.6) should be used as a rinsing buffer. PBS should never be used! Phosphates act as a competitive inhibitor to alkaline phosphatase enzymes.For optimum performance, use IMMUNOCARE TBS Wash Buffer with AP activator or TBS Wash Buffer 20X.
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This product is highly stable when compared to other chromogens. We have used this chromogen for a triple-stain with a HIER step in between different incubations, and this precipitate holds very well in both aqueous conditions, and survives xylene washes, which is more than can be said for other chromogen kits offered through other companies.